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产地 | 国产/进口 |
品牌 | xuanya |
货号 | XY-KT-2454 |
保存条件 | 在-20°C下储存一年,避免重复冻融循环 |
用途 | |
应用范围 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 |
抗原来源 | |
CAS编号 | |
保质期 | |
抗体名 | 胰岛-素受体底物-4抗体 |
是否单克隆 | |
克隆性 | 是 |
靶点 | |
适应物种 | Mouse, Rat |
形态 | Lyophilized or Liquid |
宿主 | |
标记物 | |
包装规格 | |
纯度 | % |
亚型 | IgG |
标识物 | |
浓度 | 1mg/ml% |
免疫原 | KLH conjugated synthetic peptide derived from mouse IRS-4:401-500/1216 |
是否进口 |
中文名称:IRS-4胰岛-素受体底物-4抗体
英文名称:IRS-4
别名:IRS4; 160 kDa phosphotyrosine protein; Insulin receptor substrate 4; IRS 4; PY160; IRS4_MOUSE; IRS-4; Phosphoprotein of 160 kDa; pp160.
规 格:100ul 200ul
浓 度:1mg/ml
抗体来源:Rabbit
克隆类型:polyclonal
交叉反应:Human, Mouse, Rat, Chicken, Pig
产品类型:一抗
研究领域:细胞生物 神经生物学 信号转导 细胞凋亡 糖尿病 内分泌病
分子量:137kDa
性 状:Lyophilized or Liquid
免 疫 原:KLH conjugated synthetic peptide derived from mouse IRS-4:401-500/1216
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品应用:ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500(石蜡切片需做抗原修复)
IRS-4胰岛-素受体底物-4抗体荧光标记抗体实验要点:
1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平;
2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率);
3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定;
4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存;
5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。
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IRS-4胰岛-素受体底物-4抗体 Anti-Smad4(Mothers against decapentaplegic homolog 4) Smad4抗体
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