中文名称：雌激素受体α抗体

英文名称：Anti-Estrogen Receptor alpha

别名：Estradiol receptor; Estrogen receptor alpha; Estradiol Receptor-alpha; Estrogen Receptor 1; Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER Alpha; ER; ER-alpha; ERalpha; ER[a]; Era; ESR; ESR1; ESR1_HUMAN; ESR2; ESRA; Estr; Estrogen receptor 1 (alpha); Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; Nuclear receptor subfamily 3 group A member 1; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR.  

规 格：100ul

浓    度：1mg/ml

抗体来源：Rabbit

克隆类型：Polyclonal

交叉反应：Human, Mouse, Rat

产品类型：一抗  

研究领域： 免疫学 生长因子和激素 病

分子量：66kDa

性    状：Lyophilized or Liquid

免 疫 原：KLH conjugated synthetic peptide derived from human Estrogen Receptor alpha :241-300/595 

亚    型： IgG 

纯化方法：affinity purified by Protein A

储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200（石蜡切片需做抗原修复） 

雌激素受体α抗体荧光标记抗体实验要点：

1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平；

2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率)；

3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定；

4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存；

5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。

与雌激素受体α抗体相关产品：

Anti-DLX4 (Dsital-less homebox4 isoformα/β)  兔抗DLX4抗体    

Anti-DMBT1 (Deleted in malignant brain tumor 1)  基因抗体    

Anti-Dnmt1(DNA methyltransferase 1)  DNA甲基转移酶1抗体    

Anti-Dnmt3a(cytosine-5)-methyltransferase 3A)  DNA甲基转移酶-3α抗体    

Anti-Dnmt3β (DNA (cytosine-5)-methyltransferase 3 β)  DNA甲基转移酶-3β抗体    

Anti-DPPA2 (developmental pluripotency associated gene2)  多能发育相关基因2抗体    

Anti-DR3/TNF-αR/TRAMP (death receptor 3)  TNF-α膜受体抗体/DR3 死亡受体3抗体    

Anti-DR4/APO2/TRAILR1 (Death receptor 4)  死亡受体4抗体    

Anti-DTNBP1 (Dysbindin-1)  易感基因抗体    

Anti-DVL1 (dishevelled 1)  蓬乱蛋白1抗体    

Anti-Egr-1 （Early growth response 1）  早期反应基因-1/应急反应生长基因1抗体    

Anti-Emp1 (epithelial membrane protein 1)  表皮膜蛋白1抗体    

anti-EMAb（Endometrium）  抗子宫内膜抗体    

Anti-Endostatin  内皮抑素/内皮他丁抗体    

Anti-EpCAM（Epithelial cell adhesion molecule）  上皮细胞粘附分子/表皮细胞粘附分子抗体    Anti-EPEC-IgG O (Anti-Enteropathogenic E.coli )  大肠埃希杆菌O抗体    

Anti-EphA2 R(Ephrin A2 Receptor)  酪氨酸蛋白激酶受体A2抗体    

雌激素受体α抗体   Anti-EphB2 R (Ephrin B2 Receptor)  酪氨酸蛋白激酶受体B2抗体    

Anti-ER  雌激素受体    

Anti-ERAB  内质网Aβ相关结合蛋白抗体