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产地 | 国产/进口 |
品牌 | xuanya |
保存条件 | 在-20°C下储存一年,避免重复冻融循环 |
货号 | XY-KT-1136 |
应用范围 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 |
CAS编号 | |
抗体名 | 磷酸化真核翻译起始因子4G抗体 |
克隆性 | 是 |
靶点 | |
适应物种 | Human, Mouse, Rat |
形态 | |
宿主 | |
亚型 | IgG |
标识物 | |
浓度 | 1mg/ml% |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human EIF4G1 around the phosphorylation site of Ser1238:AL(p-S)EE |
中文名称:Anti-phospho-EIF4G1(Ser1238)
英文名称:Anti-phospho-EIF4G1(Ser1238)
别名:eIF4G (phospho-Ser1238); eIF4G (phospho-S1238); p-eIF4G (Ser1238); p-eIF4G (S1238); eIF 4 gamma 1; eIF 4G 1; eIF 4G1; EIF4 gamma; EIF4F; EIF4G; EIF4GI; Eukaryotic translation initiation factor 4 gamma 1; p220; DKFZp686A1451; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; EIF4G-I; IF4G1_HUMAN.
规 格:100ul
浓 度:1mg/ml
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human, Mouse, Rat
产品类型:一抗
研究领域: 免疫学 转录调节因子
分子量:175kDa
性 状:Lyophilized or Liquid
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human EIF4G1 around the phosphorylation site of Ser1238:AL(p-S)EE
亚 型: IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品应用:ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500(石蜡切片需做抗原修复)
Anti-phospho-EIF4G1(Ser1238)荧光标记抗体实验要点:
1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平;
2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率);
3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定;
4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存;
5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。
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