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产地 | 国产/进口 |
品牌 | xuanya |
保存条件 | 在-20°C下储存一年,避免重复冻融循环 |
货号 | XY-KT-1122 |
应用范围 | WB=1:500-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:100-500 IF=1:100-500 |
CAS编号 | |
抗体名 | phospho-EGFR (Ser695) |
克隆性 | 是 |
靶点 | |
适应物种 | Human, Mouse, Rat |
形态 | |
宿主 | |
亚型 | IgG |
标识物 | |
浓度 | 1mg/ml% |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Ser695:P(p-S)GE |
中文名称:磷酸化表皮生长因子受体兔单克隆抗体phospho-EGFR (Ser695)
英文名称:phospho-EGFR (Ser695)
别 名:EGFR(S695); EGFR (phospho S695); p-EGFR (phospho S695);EGFR; Avian erythroblastic leukemia viral (v erb b) oncogene homolog; Avian erythroblastic leukemia viral (verbb) oncogene homolog; Cell growth inhibiting protein 40; Cell proliferation inducing protein 61; EGF R; EGFR; Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog); Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian); Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; HER1; mENA; Oncogene ERBB; PIG61; Receptor tyrosine protein kinase ErbB 1; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5; EGFR_HUMAN.
规 格:100ul
浓 度:1mg/ml
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human, Mouse, Rat
产品类型:一抗
研究领域: 免疫学 生长因子和激素 转录调节因子
分子量:170kDa
性 状:Lyophilized or Liquid
免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human EGFR around the phosphorylation site of Ser695:P(p-S)GE
亚 型: IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品应用:WB=1:500-1000 IHC-P=1:50-200 IHC-F=1:50-200 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
磷酸化表皮生长因子受体兔单克隆抗体phospho-EGFR (Ser695)荧光标记抗体实验要点:
1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平;
2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率);
3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定;
4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存;
5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。
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