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Anti-phospho-EIF4EBP1(Ser111)
  • 品牌:xuanya
  • 产地:国产/进口
  • 货号:XY-KT-1116
  • 发布日期: 2019-03-13
  • 更新日期: 2025-07-09
产品详请
产地 国产/进口
品牌 xuanya
保存条件 在-20°C下储存一年,避免重复冻融循环
货号 XY-KT-1116
应用范围 WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
CAS编号
抗体名 磷酸化eIF4E结合蛋白抗体
克隆性
靶点
适应物种 Human, Mouse, Rat, Dog, Cow, Horse
形态
宿主
亚型 IgG
标识物
浓度 1mg/ml%
免疫原 KLH conjugated Synthesised phosphopeptide derived from human 4EBP1 around the phosphorylation site of Ser111:EE(p-S)QF

中文名称:Anti-phospho-EIF4EBP1(Ser111)

英文名称:Anti-phospho-EIF4EBP1(Ser111)

别    名:eIF4EBP1 (phospho S111); eIF4EBP1 (phospho Ser111); p-eIF4EBP1 (Ser111); eIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E BP1; 4EBP1; BP 1; BP1; eIF4E binding protein 1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E binding protein 1; MGC4316; PHAS I; PHASI; PHAS-I; PHAS; 4E-BP1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; Phosphorylated heat and acid stable protein regulated by insulin 1; 4EBP1_HUMAN

规 格:100ul

浓    度:1mg/ml

抗体来源:Rabbit

克隆类型:Polyclonal

交叉反应:Human, Mouse, Rat, Dog, Cow, Horse

产品类型:一抗  

研究领域: 免疫学 信号转导 结合蛋白

分子量:13kDa

性    状:Lyophilized or Liquid

免 疫 原:KLH conjugated Synthesised phosphopeptide derived from human 4EBP1 around the phosphorylation site of Ser111:EE(p-S)QF  

亚    型: IgG 

纯化方法:affinity purified by Protein A

储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用:WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500(石蜡切片需做抗原修复) 

 

Anti-phospho-EIF4EBP1(Ser111)荧光标记抗体实验要点:

1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平;

2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率);

3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定;

4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存;

5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。


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