中文名称：内皮素B受体抗体ETBR

英文名称：Anti-ETBR/Endothelin B Receptor

别    名：ABCDS; Ednra; Ednrb; EDNRB_HUMAN; Endothelin B receptor; Endothelin B receptor precursor; Endothelin receptor Non selective type; Endothelin receptor non-selective type; Endothelin receptor type B; ET B; ET-B; ET-BR; ETB; ETRB; Hirschsprung disease 2; HSCR; HSCR2; EDNRB; HSCR; WS4A.

规 格：100ul

浓    度：1mg/ml

抗体来源：Rabbit

克隆类型：Polyclonal

交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit

产品类型：一抗  

研究领域：心 免疫学 神经生物学 生长因子和激素

分子量：50kDa

性    状：Lyophilized or Liquid

免 疫 原：KLH conjugated synthetic peptide derived from human EDNRB:31-130/442 <Extracellular>

亚    型： IgG 

纯化方法：affinity purified by Protein A

储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500（石蜡切片需做抗原修复） 

内皮素B受体抗体ETBR荧光标记抗体实验要点：

1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平；

2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率)；

3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定；

4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存；

5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。

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