中文名称：内质网蛋白ERp72抗体

英文名称：ERp72

别    名：Calcium binding protein intestinal related; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; ERP 70; ERp 72; ERp-72; ERP70; ERp72; PDIA 4; PDIA4; PDIA4_HUMAN; PDIR; Protein disulfide isomerase A4; Protein disulfide isomerase associated 4; Protein disulfide isomerase family A member 4; Protein disulfide isomerase related protein; Protein disulfide-isomerase A4; Protein ERp 72; Protein ERp72.  

规 格：100ul

浓    度：1mg/ml

抗体来源：Rabbit

克隆类型：Polyclonal

交叉反应：Human, Mouse, Rat, Cow, Rabbit, Sheep, Monkey

产品类型：一抗  

研究领域： 细胞生物 信号转导

分子量：71kDa

性    状：Lyophilized or Liquid

免 疫 原：KLH conjugated synthetic peptide derived from human ERp72:451-550/645  

亚    型： IgG 

纯化方法：affinity purified by Protein A

储 存 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件：Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品应用：WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500（石蜡切片需做抗原修复） 

内质网蛋白ERp72抗体荧光标记抗体实验要点：

1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平；

2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率)；

3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定；

4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存；

5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。

与内质网蛋白ERp72抗体相关产品：

Anti-HCV-HCBP1/ACY-3(Hepatitis C virus core-binding protein 1)  病毒核心蛋白结合蛋白-1抗体    

Anti-HCV-NS1  病毒-NS1抗体    

Anti-HCV-NS3  病毒-NS3抗体    

Anti-HCV-NS4a  病毒-NS4a抗体    

Anti-HDL-R（High Density Lipoprotein Receptor）  受体抗体    

Anti-Heamachrome  血红素抗体    

Anti-HEV  病毒抗体    

Anti-HGF(hepatocyte growth factor)  肝细胞生长因子抗体    

Anti-HGV（Hepatitis G vivus）  庚型肝炎病毒抗体    

Anti-HHV4/EBV（Human herpesvirus 4 type 2/epstein-barr virus ）  人类病毒4抗体    

Anti-HIV-1（human immunodeficiency virus type 1）Anti-Anti-Anti-Anti-HIV-1（human immunodeficiency virus type 1）  病毒-1/人类病毒1型抗体    

Anti-HLA-DR （human leukoyte antigen DR）  HLA-DR抗原抗体    

Anti-HLA-G (Human Leukocyte Antigen-G）  人类白细胞抗原G/组织相容性白细胞抗原G抗体    Anti-HLA-E (Human Leukocyte Antigen-E）  人类白细胞抗原E    

Anti-HMGA1 (high mobility group AT-hook 1)  高迁移率族蛋白A1抗体    

Anti-HMGA2 (high mobility group A2)  高迁移率族蛋白A2抗体    

内质网蛋白ERp72抗体 Anti-HMGB1 (High Mobility Group Box protein 1)  高迁移率族蛋白-1抗体    

Anti-HO-1/HSP32 (Hemeo xygenase 1)  血红素氧合酶-1/热休克蛋白-32抗体    

Anti-HO-2 （Heme oxygenase 2）  血红素氧合酶-2抗体