![]() |
产地 | 国产/进口 |
品牌 | xuanya |
保存条件 | 在-20°C下储存一年,避免重复冻融循环 |
货号 | XY-KT-1064 |
应用范围 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 |
CAS编号 | |
抗体名 | eRF1 |
克隆性 | 是 |
靶点 | |
适应物种 | Human, Mouse, Rat, Dog, Cow, Horse, Zebrafish, Arabidopsis Thaliana, Xenopus laevis |
形态 | |
宿主 | |
亚型 | IgG |
标识物 | |
浓度 | 1mg/ml% |
免疫原 | KLH conjugated synthetic peptide derived from human eRF1:341-437/437 |
中文名称:真核肽链释放因子1抗体eRF1
英文名称:eRF1
别 名:Cl1 protein; D5S1995; ERF; eRF1; ERF1_HUMAN; ETF1; Eukaryotic peptide chain release factor subunit 1; Eukaryotic release factor 1; Eukaryotic translation termination factor 1; MGC111066; Polypeptide chain release factor 1; Protein Cl1; RF1; Sup45 (yeast omnipotent suppressor 45) homolog like 1; SUP45L1; TB3 1; TB3-1.
规 格:100ul
浓 度:1mg/ml
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human, Mouse, Rat, Dog, Cow, Horse, Zebrafish, Arabidopsis Thaliana, Xenopus laevis
产品类型:一抗
研究领域:细胞生物 转录调节因子 表观遗传学 G蛋白信号
分子量:49kDa
性 状:Lyophilized or Liquid
免 疫 原:KLH conjugated synthetic peptide derived from human eRF1:341-437/437
亚 型: IgG
纯化方法:affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
产品应用:ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500(石蜡切片需做抗原修复)
真核肽链释放因子1抗体eRF1荧光标记抗体实验要点:
1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平;
2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率);
3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定;
4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存;
5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。
与真核肽链释放因子1抗体eRF1相关产品:
Anti-AT1R(Angiotensin II Type 1 Receptor) 紧张素Ⅱ-1型受体抗体
Anti-ATF1 (Activating Transcription Factor1) 活化复制因子1抗体
Anti-ATF2 (Activating Transcription Factor2) 活化复制因子2抗体
Anti-ATF3 (Activating Transcription Factor3) 活化转录因子3抗体
Anti-B7-H1/PDL1/CD274(programmed death ligand 1) 程序性死亡配体1抗体
Anti-B7-H4 ( V-set domain containing T cell activation inhibitor 1 ) B7H4抗体
Anti-BACE/ASP2 β分泌酶抗体
Anti-Bad (BCL-xL/BCL-2-associated death promoter) 相关死亡促进因子Bad抗体
Anti-BKCa channels(calcium-activated potassium channel) 钙激活钾通道蛋白抗体
Anti-BMP2 (bone morphogenetic protein 2) 骨形态发生蛋白-2抗体
Anti-BMP-7(bone morphogenetic protein-7) 骨形态发生蛋白-7抗体
Anti-BNP (Brain Natriuretic Peptide ) 脑钠素/利钠肽抗体
Anti-BNP/Biotin (Brain Natriuretic Peptide ) 生物素化脑钠肽
Anti-BRAF 黑素瘤相关蛋白抗体
Anti-BYTA1(Breast cancer susceptbility gene 1)(mouse rat) 易感基因1抗体(小鼠 大鼠)
Anti-BYTA1(Breast cancer susceptbility gene 1)(human) 易感基因1抗体(人)
Anti-BYTA2(Breast cancer susceptbility gene 2) 易感基因2抗体
Anti-BrdU (Bromodeoxyuridine) 溴脱氧尿苷抗体
Anti-BrdU (Bromodeoxyuridine) 溴脱氧尿苷单克隆抗体
Anti-BrdU (Bromodeoxyuridine) 溴脱氧尿苷抗体