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产地 | 国产/进口 |
品牌 | xuanya |
货号 | XY-KT-1002 |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C |
用途 | |
应用范围 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user |
抗原来源 | |
CAS编号 | |
保质期 | |
抗体名 | phospho-ECT2 (Thr373) |
是否单克隆 | |
克隆性 | 是 |
靶点 | |
适应物种 | Human, Dog, Cow, Sheep |
形态 | |
宿主 | |
标记物 | |
包装规格 | |
纯度 | % |
亚型 | IgG |
标识物 | |
浓度 | 100ul% |
免疫原 | KLH conjugated Synthesised phosphopeptide derived from human ECT2 around the phosphorylation site of Thr373:LN(P-T)PN |
是否进口 |
中文名称:磷酸化上皮细胞癌转化蛋白2抗体
英文名称:phospho-ECT2 (Thr373)
别 名:ECT2 (phospho T373); ECT2 (phospho Thr373); p-ECT2 (Thr373); ECT 2; ECT2; ECT-2; ECT2 protein; Epithelial cell transforming sequence 2; Epithelial cell transforming sequence 2 oncogene; Epithelial cell transforming sequence 2 oncogene protein; FLJ10461; MGC138291; Protein ECT2; ECT2_HUMAN; Epithelial cell-transforming sequence 2 oncogene
规 格:100ul
浓 度:1mg/ml
抗体来源:Rabbit
克隆类型:Polyclonal
交叉反应:Human, Dog, Cow, Sheep
产品类型:一抗
研究领域:肿瘤 转录调节因子 免疫学
分子量:100kDa
性 状:Lyophilized or Liquid
免 疫 原: KLH conjugated Synthesised phosphopeptide derived from human ECT2 around the phosphorylation site of Thr373:LN(P-T)PN
亚 型: IgG
纯化方法: affinity purified by Protein A
储 存 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol
产品应用:WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user
磷酸化上皮细胞癌转化蛋白2抗体产品优势:
荧光标记抗体实验要点:
1.偶联反应要求在尽可能接近pH9.8的溶液中进行,而且注意在反应过程中要保持此pH水平;
2.要确定在偶联反应缓冲液中不含游离氨基(Tris,氨及叠氮钠均可与FITC反应,因此会降低蛋白质与FITC的偶联率);
3.FITC与蛋白质的比值(F/P),可通过测定495m与280nm吸光度来鉴定;
4.FITC缺限是易被光淬灭,因此复合物必须始终避光保存;
5.如果FITC一复合物对PBS透析不充分,可能造成高本底,对免疫荧光染色产生干扰。
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